Takizawa T, Saito T
Department of Anatomy, Jichi Medical School, Tochigi, Japan.
J Electron Microsc (Tokyo). 1996 Jun;45(3):242-6. doi: 10.1093/oxfordjournals.jmicro.a023440.
We present a novel freeze-fracture cytochemistry method based upon enzyme cytochemistry. By this method, freeze-fractured membranes are labeled with cerium as an enzyme cytochemical marker on replicas. The cerium capture method was suitable for freeze-fracture enzyme cytochemistry because the cerium phosphate reaction product is stable after replica-cleaning. As a model system, acid phosphatase, which is a well-known lysosomal marker, was detected on freeze-fractured membranes of lysosomes in the proximal tubular epithelium of the rat kidney. This technique should be a useful addition for analyzing the ultrastructure of biological membranes.
我们提出了一种基于酶细胞化学的新型冷冻断裂细胞化学方法。通过这种方法，冷冻断裂的膜在复制品上用铈作为酶细胞化学标记物进行标记。铈捕获法适用于冷冻断裂酶细胞化学，因为磷酸铈反应产物在复制品清洗后是稳定的。作为一个模型系统，在大鼠肾脏近端小管上皮细胞溶酶体的冷冻断裂膜上检测到了一种著名的溶酶体标记物——酸性磷酸酶。这项技术对于分析生物膜的超微结构应该是一个有用的补充。
