Ras相关GTP酶的ARF家族新成员（ARL5）的克隆

Cloning of a novel member (ARL5) of the ARF-family of Ras-related GTPases.

作者信息

Breiner M, Schürmann A, Becker W, Joost H G

机构信息

Institut für Pharmakologie und Toxikologie, RWTH Aachen, Germany.

出版信息

Biochim Biophys Acta. 1996 Jul 31;1308(1):1-6. doi: 10.1016/0167-4781(96)00081-4.

DOI:10.1016/0167-4781(96)00081-4

PMID:8765741

Abstract

A novel ras-related GTPase with a unique structure was cloned by PCR-amplification with degenerate primers and screening of a rat fat cell cDNA library. The deduced amino acid sequence of the cDNA comprises all 6 GTP binding motifs which are conserved in Ras-related GTPases. The sequence is similar to that of ADP-ribosylation factors (ARF), and shows several structural features typical for the ARF-family. Because its closest relative is the GTPase ARL1 (49% identical amino acids, 54% identical nucleotides within the coding region), the protein was designated ARL5 (ARF-like protein 5). Low amounts of mRNA were found in most rat tissues examined (heart, skeletal muscle, fat, liver, kidney, lung, spleen, intestine, testis, and thymus) with highest levels in brain, intestine, and thymus.

摘要

通过使用简并引物进行PCR扩增并筛选大鼠脂肪细胞cDNA文库，克隆出一种具有独特结构的新型ras相关GTP酶。该cDNA推导的氨基酸序列包含在Ras相关GTP酶中保守的所有6个GTP结合基序。该序列与ADP核糖基化因子（ARF）的序列相似，并显示出ARF家族典型的几个结构特征。由于其最密切的亲属是GTP酶ARL1（编码区内49%的氨基酸相同，54%的核苷酸相同），因此该蛋白质被命名为ARL5（ARF样蛋白5）。在大多数检测的大鼠组织（心脏、骨骼肌、脂肪、肝脏、肾脏、肺、脾脏、肠道、睾丸和胸腺）中发现了少量mRNA，在脑、肠道和胸腺中的水平最高。