Brito B, Palacios J M, Ruiz-Argüeso T, Imperial J
Laboratorio de Microbiología. Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Spain.
Biochim Biophys Acta. 1996 Jul 31;1308(1):7-11. doi: 10.1016/0167-4781(96)00083-8.
The 4 kb DNA region located immediately upstream of the Rhizobium leguminosarum bv. viciae UPM791 hydrogen structural genes was sequenced and found to encode a chemoreceptor of the methyl-accepting type, the first to be described in a rhizobial symbiotic plasmid. Two additional open reading frames were found. Their protein products showed sequence homology to dehydrogenases and isomerases involved in the metabolism of aromatic compounds. Mutant analysis showed that this region is not required for hydrogenase activity.
对豌豆根瘤菌蚕豆生物变种UPM791氢化酶结构基因上游紧邻的4 kb DNA区域进行了测序，发现其编码一种甲基受体型化学感受器，这是在根瘤菌共生质粒中首次描述的此类感受器。还发现了另外两个开放阅读框。它们的蛋白质产物与参与芳香族化合物代谢的脱氢酶和异构酶具有序列同源性。突变分析表明，该区域对于氢化酶活性并非必需。
