Bockhardt A, Krause I, Klostermeyer H
Forschungszentrum für Milch und Lebensmittel, Technische Universität München, Freising-Weihenstephan, Germany.
Z Lebensm Unters Forsch. 1996 Jul;203(1):65-70. doi: 10.1007/BF01267772.
An automated derivatization with dabsyl chloride in combination with a high-performance liquid chromatographic analysis is described for the determination of biogenic amines in complex matrices. The sample clean-up procedure consists of an ultrafiltration step, resulting in average recoveries for cheese in the range of 88% to 100%. A linear relation between the area of the peak and amine concentration was observed between 0.5 and 500 pmol for all amines under investigation and the detection limits ranged between 0.34 and 0.76 pmol. The average repeatability of both the performance of the chromatographic determination and the whole method, including sample preparation, examined using cheese samples containing different amounts of biogenic amines was found to be between 2.0% and 3.7%. The method was applied to the analysis of several types of food and feed, selected examples are given by the separation of dabsyl derivates from cheese, wine, and salami-type sausage.
本文描述了一种使用丹磺酰氯进行自动衍生化并结合高效液相色谱分析的方法，用于测定复杂基质中的生物胺。样品净化程序包括超滤步骤，奶酪的平均回收率在88%至100%之间。在所研究的所有胺类中，在0.5至500皮摩尔之间观察到峰面积与胺浓度之间呈线性关系，检测限在0.34至0.76皮摩尔之间。使用含有不同量生物胺的奶酪样品对色谱测定性能和整个方法（包括样品制备）进行检测，发现平均重复性在2.0%至3.7%之间。该方法应用于几种食品和饲料的分析，从奶酪、葡萄酒和萨拉米香肠中分离丹磺酰衍生物给出了选定的示例。
