使用酵母DNA Alu-PCR产物进行竞争，通过荧光原位杂交技术将22个酵母人工染色体定位到人类染色体上。

Mapping of 22 YACs on human chromosomes by fish using yeast DNA Alu-PCR products for competition.

作者信息

Goguel A F, Pulcini F, Danglot G, Fauvet D, Devignes M D, Bernheim A

机构信息

Laboratoire de Cytogénetique et Génétique Oncologiques, CNRS URA 1967, Institut Gustave Roussy, Villejuif, France.

出版信息

Ann Genet. 1996;39(2):64-8.

PMID:8766135

Abstract

Chromosomal assignment and analysis of chimerism of 22 YACs was performed by FISH. Probes were obtained by PCR amplification of the human YAC inserts with Alu primers. Maximum amplification of various inter-Alu elements was obtained when the primer annealing temperature was below the optimal temperature needed for high specificity. In these conditions, yeast DNA contributed to the amplification of various Alu-PCR products and, since strong competition was required for the suppression of all Alu sequences, yeast Alu-PCR products fulfilled this purpose efficiently.

摘要

通过荧光原位杂交（FISH）对22个酵母人工染色体（YAC）进行染色体定位和嵌合体分析。探针通过用Alu引物对人YAC插入片段进行PCR扩增获得。当引物退火温度低于高特异性所需的最佳温度时，可实现各种Alu元件间的最大扩增。在这些条件下，酵母DNA有助于各种Alu-PCR产物的扩增，并且由于抑制所有Alu序列需要强烈竞争，酵母Alu-PCR产物有效地实现了这一目的。