de Souza A P, Allamand V, Richard I, Brenguier L, Chumakov I, Cohen D, Beckmann J S
Universidade Estadual de Campinas, (SP), Brazil.
Genomics. 1994 Jan 15;19(2):391-3. doi: 10.1006/geno.1994.1079.
Primary genetic maps based on highly informative markers are now available. The local density of these markers may, however, not be sufficient. There is thus a need for new means to generate polymorphic markers from targeted regions of the genome. This can be achieved by selectively cloning and sequencing (CA)n-positive human inter-Alu sequences from targeted YAC clones. This method was tested on 21 YACs and led to the development of seven new polymorphic microsatellite markers.
基于高信息含量标记的初级遗传图谱现已可用。然而，这些标记的局部密度可能不足。因此，需要新的方法从基因组的目标区域产生多态性标记。这可以通过从目标酵母人工染色体（YAC）克隆中选择性克隆和测序（CA）n阳性人类Alu间序列来实现。该方法在21个YAC上进行了测试，并产生了7个新的多态性微卫星标记。
