Di Luca M, Pastorino L, Raverdino V, De Graan P N, Caputi A, Gispen W H, Cattabeni F
Institute of Pharmacological Sciences, University of Milano, Italy.
FEBS Lett. 1996 Jul 8;389(3):309-13. doi: 10.1016/0014-5793(96)00612-6.
Electrospray mass spectrometry coupled to liquid chromatography was utilized to measure two PKC neuronal substrates, B-50/GAP-43 and neurogranin, in single rat brain areas. Aliquots of perchloric acid extracts were directly injected and mass spectra recorded. At elution times of 14.2 and 27.0 min two molecular species of MW 7450 and 23 602 Da were observed. These values are in excellent agreement for the expected MW for rat neurogranin and B-50/GAP-43. The presence of molecular species shifted by 80 mass units in both cases indicates that these proteins are present in phosphorylated forms in cortical and hippocampal extracts.
采用液相色谱-电喷雾质谱联用技术测定大鼠单个脑区中的两种蛋白激酶C神经元底物，即B-50/GAP-43和神经颗粒素。取高氯酸提取物的等分试样直接进样，并记录质谱图。在洗脱时间为14.2分钟和27.0分钟时，观察到分子量分别为7450道尔顿和23602道尔顿的两种分子种类。这些数值与大鼠神经颗粒素和B-50/GAP-43的预期分子量高度吻合。两种情况下均出现了分子量偏移80个质量单位的分子种类，这表明这些蛋白质在皮质和海马提取物中以磷酸化形式存在。
