Onoda A, Kobayashi Y, Hoshino S
Faculty of Bioresources, Mie University, Japan.
Reprod Nutr Dev. 1996;36(3):311-20. doi: 10.1051/rnd:19960308.
The amino acid requirements of Neocallimastix sp N 13 isolated from sheep rumen were studied using supplements containing either ten essential amino acids (10 EAA) or eight nonessential amino acids (8 NEAA), or both (18 AA). Although the fungus could grow in a medium containing ammonium sulphate as the sole source of nitrogen, amino acid supplements, especially 18 AA, greatly stimulated its growth. Omission of the sulphur-containing amino acids (SCAA) from the 18 AA mixture markedly reduced the growth-stimulating effect, and sulphide, but not sulphate, substituted for SCAA only partly compensated for this omission. Omission of aromatic, branched chain, basic, acidic, aliphatic and hydroxy amino acids, and proline, singly or in combination, all reduced fungal growth to some degree as compared with the 18 AA supplement. A three amino acid (leucine, methionine and histidine) supplement was a potent stimulator for the fungus, whereas another three amino acid combination (glutamic acid, methionine and serine) was ineffective. The results indicated that Neocallimastix sp N 13 effectively utilized amino acids for its growth, but the requirements for the different amino acids differed from those of N patriciarum.
利用含有十种必需氨基酸（10种EAA）、八种非必需氨基酸（8种NEAA）或两者皆有（18种AA）的补充剂，研究了从绵羊瘤胃中分离出的新丽鞭毛虫N 13的氨基酸需求。尽管该真菌能够在以硫酸铵作为唯一氮源的培养基中生长，但氨基酸补充剂，尤其是18种AA的补充剂，极大地促进了其生长。从18种AA混合物中去除含硫氨基酸（SCAA）显著降低了生长促进作用，并且硫化物而非硫酸盐替代SCAA只能部分弥补这种去除的影响。与18种AA补充剂相比，单独或组合去除芳香族、支链、碱性、酸性、脂肪族和羟基氨基酸以及脯氨酸，均在一定程度上降低了真菌生长。三种氨基酸（亮氨酸、蛋氨酸和组氨酸）的补充剂是该真菌的有效刺激物，而另一种三种氨基酸组合（谷氨酸、蛋氨酸和丝氨酸）则无效。结果表明，新丽鞭毛虫N 13有效地利用氨基酸进行生长，但其对不同氨基酸的需求与派氏新丽鞭毛虫不同。
