Eckmiller M S
C. & O. Vogt Institut für Hirnforschung, Medizinische Einrichtungen der Heinrich-Heine-Universität Düsseldorf, Postfach 101007, D-40001 Düsseldorf, Germany.
Cell Tissue Res. 1996 Jul;285(1):165-9. doi: 10.1007/s004410050632.
The ciliary axoneme in photoreceptors from the retina of Xenopus laevis was examined by immunofluorescent staining of tubulin throughout the light/dark cycle. The immunofluorescent axoneme extended along only part of the length of rod outer segments but the entire length of cone outer segments. Both the cone axoneme and outer segment elongated during the day and shortened (presumably by shedding) during the night. Fragments of immunofluorescent axonemes were found within packets of outer segment material breaking off from cone tips. These findings show that the ciliary axoneme in the Xenopus retina is replaced during the renewal of outer segments in cones. No evidence has been found for renewal of the axoneme in rods, and thus the stability of the ciliary axoneme may differ in rod and cone photoreceptors.
通过在整个光/暗周期中对微管蛋白进行免疫荧光染色，研究了非洲爪蟾视网膜光感受器中的睫状轴丝。免疫荧光轴丝仅沿着视杆细胞外段长度的一部分延伸，但沿着视锥细胞外段的全长延伸。视锥细胞的轴丝和外段在白天都会延长，而在夜间会缩短（可能是通过脱落）。在从视锥细胞尖端脱落的外段物质包中发现了免疫荧光轴丝的片段。这些发现表明，非洲爪蟾视网膜中的睫状轴丝在视锥细胞外段更新过程中会被替换。未发现视杆细胞轴丝更新的证据，因此睫状轴丝在视杆细胞和视锥细胞光感受器中的稳定性可能不同。
