Capillary isoelectric focusing as a tool in the examination of antibodies, peptides and proteins of pharmaceutical interest.

This paper describes the recent history and development of capillary isoelectric focusing (cIEF), as it has evolved over the past 10 years forming a distinct mode of high-performance capillary electrophoresis (HPCE). The theory, equations, fundamentals and basics of cIEF are discussed and described, including modes of focusing and mobilization, coated vs uncoated capillaries, different detection schemes, resolutions possible, peak capacity possible and final commercialized approaches now available. Then, the applications of the technique are emphasized, as applied to smaller peptides, larger proteins and still larger antibodies and antibody-protein complexes. The emphasis has been on the application of capillary electromigration techniques in drug analysis. Throughout, attempts have been made to emphasize the potential applications and uses of cIEF methods, and how these might be successfully utilized in drug analysis and assays for larger biopolymers.