Büscher U, Hertwig K, Dudenhausen J W
Abteilung für Geburtsmedizin und Arbeltsgruppe Perinatale Medizin, Virchow Klinikum, Medizinische Fakultät, Humboldt-Universität zu Berlin.
Geburtshilfe Frauenheilkd. 1996 May;56(5):243-7. doi: 10.1055/s-2007-1022268.
To investigate the concentrations of amniotic fluid erythropoietin in normal and risk pregnancies.
The concentrations of erythropoietin were measured in 150 samples of amniotic fluid. The samples were obtained by amniocentesis and amniotomia and were analysed using an enzyme-linked immunosorbent assay (ELISA). Results were available within 6 hours. The intra-assay variation was 6.4%, the inter-assay variation 7.2%.
The range of erythropoietin concentration in all samples was between 0.23 and 80 U/L and in a defined group of normal pregnancies between 1.20 and 6.53 U/L (10%-90% percentile). Correlation was found between the concentration of erythropoietin and maternal hypertension (p = 0.0159), amnion infection syndrome in combination with premature birth (p = 0.0593), fetal growth retardation (p = 0.784), and base-excess (p = 0.0487). Elevated erythropoietin concentrations were found in a defined risk group with Apgar scores below 7 after 1 minute (p = 0.072) and after 5 minutes (p = 0.0037). There is a connection between postpartal transfer to the intensive-care unit and elevated erythropoietin concentrations (p = 0.073). No influence is exercised by the child's sex on the concentration of erythropoietin. No significant connection was found between the level of erythropoietin and smoking during pregnancy, volume of amniotic fluid and maturity at birth. A critical erythropoietin concentration could be postulated at 12 U/L. Children with higher levels showed heavy and severe disorders.
Elevated erythropoietin levels in amniotic fluid indicate prolonged fetal hypoxaemia. Using the ELISA-technique a rapid prepartal determination of such situations is possible and might be helpful in the clinical procedure.
研究正常妊娠和高危妊娠中羊水促红细胞生成素的浓度。
检测150份羊水样本中促红细胞生成素的浓度。样本通过羊膜穿刺术和羊膜切开术获取，并采用酶联免疫吸附测定法（ELISA）进行分析。6小时内可获得结果。批内变异为6.4%，批间变异为7.2%。
所有样本中促红细胞生成素浓度范围为0.23至80 U/L，在特定的正常妊娠组中为1.20至6.53 U/L（第10百分位数至第90百分位数）。发现促红细胞生成素浓度与母亲高血压（p = 0.0159）、羊膜感染综合征合并早产（p = 0.0593）、胎儿生长受限（p = 0.784）和碱剩余（p = 0.0487）之间存在相关性。在特定的高危组中，出生后1分钟Apgar评分低于7分（p = 0.072）和5分钟后（p = 0.0037）时，促红细胞生成素浓度升高。产后转入重症监护病房与促红细胞生成素浓度升高之间存在关联（p = 0.073）。孩子的性别对促红细胞生成素浓度无影响。未发现促红细胞生成素水平与孕期吸烟、羊水量和出生成熟度之间存在显著关联。可假定促红细胞生成素的临界浓度为12 U/L。浓度较高的儿童表现出严重和重度疾病。
羊水中促红细胞生成素水平升高表明胎儿长期缺氧。使用ELISA技术可在产前快速确定此类情况，这可能有助于临床操作。
