蜡样芽孢杆菌产生另一种肠毒素复合物的证据。
Evidence for a further enterotoxin complex produced by Bacillus cereus.
作者信息
Granum P E, Andersson A, Gayther C, te Giffel M, Larsen H, Lund T, O'Sullivan K
机构信息
Department of Pharmacology, Microbiology and Food Hygiene, Norwegian College of Veterinary Medicine, Oslo, Norway.
出版信息
FEMS Microbiol Lett. 1996 Aug 1;141(2-3):145-9. doi: 10.1111/j.1574-6968.1996.tb08376.x.
Out of 321 strains of Bacillus cereus from several sources and isolated in four different countries, 239 (74%) produced cytotoxins. Only 127 (53%) of the cytotoxic strains were positive for the B-component gene of the haemolysin BL (enterotoxin) by polymerase chain reaction (PCR). Western blots using antiserum produced against enterotoxin(s) gave positive results for 199 (83%) of the cytotoxic B. cereus strains. On closer examination of seven of the strains, involved in food poisoning, we found that two strains completely lacked the L2- and B-components (of the haemolysin BL), and two strains were negative for the B-component gene by PCR, but were positive for the L2-component. From our experiments we concluded that there is at least one enterotoxin complex in addition to the haemolysin BL enterotoxin and enterotoxin T.
从多个来源、在四个不同国家分离得到的321株蜡样芽孢杆菌中，239株（74%）产生细胞毒素。通过聚合酶链反应（PCR），仅127株（53%）产生细胞毒素的菌株的溶血素BL（肠毒素）的B组分基因呈阳性。使用针对肠毒素产生的抗血清进行的蛋白质印迹分析显示，199株（83%）产生细胞毒素的蜡样芽孢杆菌菌株呈阳性结果。在仔细检查涉及食物中毒的7株菌株时，我们发现有两株菌株完全缺乏（溶血素BL的）L2和B组分，两株菌株的B组分基因通过PCR检测呈阴性，但L2组分呈阳性。从我们的实验中我们得出结论，除了溶血素BL肠毒素和肠毒素T之外，至少还存在一种肠毒素复合物。
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