Carlson C R, Johansen T, Kolstø A B
Institute of Pharmacy and Biotechnology Centre of Oslo, University of Oslo, Norway.
FEMS Microbiol Lett. 1996 Aug 1;141(2-3):163-7. doi: 10.1111/j.1574-6968.1996.tb08379.x.
A physical map of the Bacillus thuringiensis subsp. canadensis HD224 chromosome based on AscI, NotI, and SfiI restriction sites has been established. The chromosome map of 4.3 Mb was similar to a revised map of the chromosome of the B. cereus type strain ATCC 14579, except that the B. thuringiensis subsp. canadensis HD224 chromosome lacked a NotI site and had two additional AscI sites. The positions of 27 probes were identical in the common macromap. A probe for the insecticidal toxin gene, cryIA, hybridized only to the B. thuringiensis subsp. canadensis HD224 chromosome. The BssHII ribotype patterns were almost identical confirming the similarity between the two strains.
已构建了基于AscI、NotI和SfiI限制酶切位点的苏云金芽孢杆菌加拿大亚种HD224染色体物理图谱。4.3 Mb的染色体图谱与蜡样芽孢杆菌模式菌株ATCC 14579的修订染色体图谱相似，不同之处在于苏云金芽孢杆菌加拿大亚种HD224染色体缺少一个NotI位点，且有另外两个AscI位点。在共同的宏观图谱中，27个探针的位置相同。一种用于杀虫毒素基因cryIA的探针仅与苏云金芽孢杆菌加拿大亚种HD224染色体杂交。BssHII核糖体型模式几乎相同，证实了这两个菌株之间的相似性。
