Schramm G, Petersen A, Bufe A, Schlaak M, Becker W M
Research Institut Borstel, Germany.
Int Arch Allergy Immunol. 1996 Aug;110(4):354-63. doi: 10.1159/000237328.
Although extract of velvet grass pollen is an important ingredient of commercial allergen extracts for immunotherapy, it is not defined concerning its major allergenic components. We have investigated the extract of velvet grass pollen by Western blotting using the sera of 97 patients and identified two major allergens of 34 and 30 kD. By two-dimensional immunoblotting with monoclonal antibodies and lectins, and by biochemical analysis the 34-kD protein was identified as a group I grass pollen allergen and the 30-kD protein as a group V grass pollen allergen. According to the nomenclature for grass pollen allergens we designated these allergens Hol l 1 and Hol l 5, respectively. Fragmentation of the velvet grass pollen extract by CNBr provided the first information on the localization of IgE binding epitopes on these two allergens. With Hol l 1 and Hol l 5 we identified two important components of commercial allergen extracts.
尽管绒毛草花粉提取物是用于免疫治疗的商业过敏原提取物的重要成分，但其主要致敏成分尚未明确。我们使用97例患者的血清通过蛋白质印迹法研究了绒毛草花粉提取物，并鉴定出两种主要过敏原，分子量分别为34 kD和30 kD。通过用单克隆抗体和凝集素进行二维免疫印迹以及生化分析，34-kD蛋白被鉴定为I组草花粉过敏原，30-kD蛋白被鉴定为V组草花粉过敏原。根据草花粉过敏原的命名法，我们分别将这些过敏原命名为Hol l 1和Hol l 5。用溴化氰对绒毛草花粉提取物进行片段化，首次提供了这两种过敏原上IgE结合表位定位的信息。通过Hol l 1和Hol l 5，我们鉴定出了商业过敏原提取物的两种重要成分。
