Runic R, Lockwood C J, Ma Y, Dipasquale B, Guller S
Department of Obstetrics and Gynecology, New York University Medical Center, New York 10016, USA.
J Clin Endocrinol Metab. 1996 Aug;81(8):3119-22. doi: 10.1210/jcem.81.8.8768884.
Recent data indicated that local production of Fas ligand (FasL) by cells of the eye and testis may confer immune tolerance at these sites. In the present study, we examined the in vivo and in vitro patterns of expression of FasL in the human placenta to provide a potential mechanism through which the fetus is afforded protection against the cytolytic actions of lymphocytes present within maternal decidua across gestation. Immunohistochemical staining of first trimester human placental tissue sections revealed the presence of FasL in cytotrophoblasts in free floating villi, anchoring villi, and cytotrophoblastic islands. FasL staining was also pronounced in syncytiotrophoblasts of term placenta indicating that FasL expression is maintained across gestation. Multiple molecular forms of FasL, suggestive of altered patterns of glycosylation, were detected in extracts of term placenta, amnion and chorion by Western blotting. In addition, in vitro expression of FasL was demonstrated to increase 2 to 3-fold during differentiation of primary cultures of cytotrophoblasts isolated from human term placentas. Local production of FasL by human cytotrophoblasts provides a mechanism through which cytotrophoblasts may induce immune tolerance and self-regulate survival during invasion and subsequent placentation.
近期数据表明，眼睛和睾丸细胞局部产生的Fas配体（FasL）可能赋予这些部位免疫耐受。在本研究中，我们检测了人胎盘中FasL的体内和体外表达模式，以提供一种潜在机制，通过该机制胎儿在整个妊娠期免受母体蜕膜内淋巴细胞的细胞溶解作用。孕早期人胎盘组织切片的免疫组织化学染色显示，游离绒毛、固定绒毛和细胞滋养层岛中的细胞滋养层细胞存在FasL。足月胎盘的合体滋养层细胞中FasL染色也很明显，表明FasL表达在整个妊娠期都得以维持。通过蛋白质印迹法在足月胎盘、羊膜和绒毛膜提取物中检测到多种FasL分子形式，提示糖基化模式改变。此外，从人足月胎盘中分离的细胞滋养层原代培养物在分化过程中，FasL的体外表达增加了2至3倍。人细胞滋养层细胞局部产生FasL提供了一种机制，通过该机制细胞滋养层细胞在侵袭和随后的胎盘形成过程中可能诱导免疫耐受并自我调节存活。
