Filipek A, Wojda U
Department of Muscle Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland.
Biochem Biophys Res Commun. 1996 Aug 5;225(1):151-4. doi: 10.1006/bbrc.1996.1145.
The distribution of calcyclin in some chicken tissues was studied by Western blotting using polyclonal antibodies raised against calcyclin purified from chicken gizzard. The protein was found in gizzard muscle and in a lesser amount in skeletal and cardiac muscle. No immunological reaction was observed in chicken liver. Immunohistochemical studies of chicken gizzard tissue revealed the presence of calcyclin only in muscle fibers. Ca(2+)-dependent interaction of chicken gizzard calcyclin with potential protein targets was also examined. By gel overlay method it was found that calcyclin bound to three proteins with molecular masses of approximately 35 kDa, 25 kDa and 15 kDa present in the cytosolic fraction derived from chicken gizzard muscle. The chicken gizzard calcyclin was also shown to interact with lysozyme.
利用针对从鸡砂囊纯化的钙周期蛋白制备的多克隆抗体，通过蛋白质免疫印迹法研究了钙周期蛋白在一些鸡组织中的分布情况。该蛋白在砂囊肌肉中被发现，在骨骼肌和心肌中的含量较少。在鸡肝脏中未观察到免疫反应。对鸡砂囊组织的免疫组织化学研究表明，钙周期蛋白仅存在于肌纤维中。还检测了鸡砂囊钙周期蛋白与潜在蛋白质靶点的钙依赖性相互作用。通过凝胶覆盖法发现，钙周期蛋白与存在于鸡砂囊肌肉细胞质组分中的三种分子量分别约为35 kDa、25 kDa和15 kDa的蛋白质结合。鸡砂囊钙周期蛋白还显示与溶菌酶相互作用。
