垂体切除的大鼠中ob mRNA的减少不能通过生长激素（GH）恢复，但会被外源性给予的胰岛素样生长因子（IGF）I进一步抑制。

Reduced ob mRNA in hypophysectomised rats is not restored by growth hormone (GH), but further suppressed by exogenously administered insulin-like growth factor (IGF) I.

作者信息

Böni-Schnetzler M, Gosteli-Peter M A, Moritz W, Froesch E R, Zapf J

机构信息

Department of Medicine, University Hospital, Zürich, Switzerland. +

出版信息

Biochem Biophys Res Commun. 1996 Aug 5;225(1):296-301. doi: 10.1006/bbrc.1996.1169.

DOI:10.1006/bbrc.1996.1169

PMID:8769133

Abstract

To examine whether GH and IGF-I participate in the regulation of obese (ob) mRNA expression we determined ob mRNA levels in epididydimal fat pads of hypophysectomised (hypox) rats, hypox rats treated with recombinant human (rh) GH or rhIGF-I and normal, weight-matched controls. We found that 1. ob mRNA was markedly suppressed after hypophysectomy (37 +/- 25% of controls), 2. GH infusion had no effect on ob mRNA, but stimulated IGF-I mRNA in fat pads, 3. IGF-I treatment further suppressed ob mRNA (3.5% +/- 0.6% of controls) and 4. serum insulin levels were decreased in all hypox groups (11.2 to 15.9% of controls). In conclusion, exogenous and GH-induced IGF-I differ in their effects on ob mRNA expression and GH is unable to restore ob mRNA towards normal at low insulin levels.

摘要

为了研究生长激素（GH）和胰岛素样生长因子-I（IGF-I）是否参与肥胖（ob）mRNA表达的调节，我们测定了垂体切除（hypox）大鼠、接受重组人（rh）GH或rhIGF-I治疗的hypox大鼠以及体重匹配的正常对照大鼠附睾脂肪垫中的ob mRNA水平。我们发现：1. 垂体切除后ob mRNA显著受到抑制（为对照的37±25%）；2. 输注GH对ob mRNA无影响，但刺激了脂肪垫中IGF-I mRNA的表达；3. IGF-I治疗进一步抑制了ob mRNA（为对照的3.5%±0.6%）；4. 所有hypox组的血清胰岛素水平均降低（为对照的11.2%至15.9%）。总之，外源性和GH诱导的IGF-I对ob mRNA表达的影响不同，并且在低胰岛素水平时GH无法使ob mRNA恢复至正常水平。