Friedman J R, Fredericks W J, Jensen D E, Speicher D W, Huang X P, Neilson E G, Rauscher F J
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA.
Genes Dev. 1996 Aug 15;10(16):2067-78. doi: 10.1101/gad.10.16.2067.
The KRAB repression domain is one of the most widely distributed transcriptional effector domains yet identified, but its mechanism of repression is unknown. We have cloned a corepressor, KAP-1, which associates with the KRAB domain but not with KRAB mutants that have lost repression activity. KAP-1 can enhance KRAB-mediated repression and is a repressor when directly tethered to DNA. KAP-1 contains a RING finger, B boxes, and a PHD finger; the RING-B1-B2 structure is required for KRAB binding and corepression. We propose that KAP-1 may be a universal corepressor for the large family of KRAB domain-containing transcription factors.
KRAB抑制结构域是迄今已鉴定出的分布最为广泛的转录效应结构域之一，但其抑制机制尚不清楚。我们克隆了一种共抑制因子KAP-1，它与KRAB结构域相关联，但不与失去抑制活性的KRAB突变体相关联。KAP-1可以增强KRAB介导的抑制作用，并且当直接与DNA相连时它就是一种阻遏物。KAP-1包含一个环状结构域、B框和一个植物同源结构域；KRAB结合和共抑制作用需要环状-B1-B2结构。我们提出，KAP-1可能是含KRAB结构域的转录因子大家族的一种通用共抑制因子。
