Wenzel A, Villa M, Mohler H, Benke D
Institute of Pharmacology, ETH, Zurich, Switzerland.
J Neurochem. 1996 Mar;66(3):1240-8. doi: 10.1046/j.1471-4159.1996.66031240.x.
The regional and developmental expression of NMDA receptors containing the NR2D subunit was analyzed on the level of the subunit mRNA and protein in rat brain. RNase protection experiments indicated that among two proposed splice variants of the NR2D subunit, only the NR2D-2 subunit is expressed. The regional distribution of the NR2D subunit protein was visualized with a newly developed NR2D-2 subunit-specific antiserum on brain sections using the histoblot technique. In adult brain, NR2D immunoreactivity was mainly restricted to diencephalic, mesencephalic, and brainstem structures. During postnatal development, the NR2D subunit was detected transiently in certain regions, such as the ventrobasal complex of the thalamus, hippocampus, inferior colliculus, and brainstem reticular formation, suggesting that NR2D subunit-containing receptors play a role in these brain areas only during development. The level of NR2D subunit mRNA and protein decreased during late postnatal development. However, significant levels of NR2D subunit mRNA and protein were present in adulthood, in particular, in the globus pallidus, thalamus, subthalamic nuclei, and superior colliculus. These results indicate a functional relevance for NMDA receptors containing the NR2D subunit in the developing and adult brain, although its expression in the adult brain is less prominent and restricted to a few brain areas.
在大鼠脑内，从亚基mRNA和蛋白质水平分析了含NR2D亚基的NMDA受体的区域及发育表达情况。核糖核酸酶保护实验表明，在NR2D亚基的两种推测剪接变体中，只有NR2D-2亚基表达。使用组织印迹技术，用新研制的NR2D-2亚基特异性抗血清在脑切片上观察NR2D亚基蛋白的区域分布。在成年脑中，NR2D免疫反应性主要局限于间脑、中脑和脑干结构。在出生后发育过程中，NR2D亚基在某些区域短暂出现，如丘脑腹侧基底复合体、海马、下丘和脑干网状结构，这表明含NR2D亚基的受体仅在发育过程中在这些脑区发挥作用。出生后晚期发育过程中，NR2D亚基mRNA和蛋白质水平下降。然而，成年期仍存在显著水平的NR2D亚基mRNA和蛋白质，特别是在苍白球、丘脑、丘脑底核和上丘。这些结果表明，含NR2D亚基的NMDA受体在发育中和成年脑中具有功能相关性，尽管其在成年脑中的表达不太显著且局限于少数脑区。
