在含有聚乙二醇的溶液中的一种双链RNA的紧密形式。
A compact form of double-stranded RNA in solutions containing poly(ethyleneglycol).
作者信息
Evdokimov Y M, Pyatigorskaya T L, Kadikov V A, Polyvtsev O F, Doskocil J, Koudelka J, Varshavsky Y M
出版信息
Nucleic Acids Res. 1976 Jun;3(6):1533-47. doi: 10.1093/nar/3.6.1533.
Molecules of single-stranded ribosomal RNA and double-stranded replicative form of phage f2 RNA (dsRNA) adopt a compact form in solutions, containing sufficiently high concentrations of salt (NaCl) and polymer (PEG). However, only in the cases of native dsRNA molecules the compact particles are characterized by a regular internal structure, which accounts for the appearance of an intense positive band in CD spectra. Heating or acidification of PEG-containing solutions of dsRNA leads to the disappearance of the intense positive CD band, which results from the "destruction" of the regular internal structure of compact particles. Comparison of properties of DNA and dsRNA compact particles formed in PEG-containing water-salt solutions suggests the existence of similar mechanisms of compactization of double-stranded polynucleotides.
单链核糖体RNA分子以及噬菌体f2 RNA的双链复制形式（dsRNA）在含有足够高浓度盐（NaCl）和聚合物（PEG）的溶液中会呈现紧密结构。然而，只有天然dsRNA分子的紧密颗粒具有规则的内部结构，这导致了圆二色光谱（CD光谱）中出现强烈的正峰。对含有PEG的dsRNA溶液进行加热或酸化会导致CD光谱中强烈正峰消失，这是由于紧密颗粒规则内部结构的“破坏”所致。对在含PEG的水盐溶液中形成的DNA和dsRNA紧密颗粒的性质比较表明，双链多核苷酸存在类似的紧密化机制。
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