滑膜中早期补体成分C3和C4的调节
Regulation of early complement components C3 and C4 in the synovium.
作者信息
Collins T, Winkelstein J A, Sullivan K E
机构信息
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
出版信息
Clin Diagn Lab Immunol. 1996 Jan;3(1):5-9. doi: 10.1128/cdli.3.1.5-9.1996.
To determine the cytokine inducibility of early complement component (C3 and C4) expression in the synovium, explant tissue was maintained in culture for 7 days. C3 and C4 production was measured by specific enzyme-linked immunosorbent assay, and RNA was evaluated by semiquantitative PCR. The effects of leukemia inhibitory factor (LIF), gamma interferon (IFN-gamma), IFN-alpha, and estrogen on C3 and C4 expression were evaluated. C3 levels were unaffected by 7 days of LIF, IFN-gamma, or IFN-alpha treatment. In contrast, C4 levels were significantly induced in synovial samples treated for 7 days with either IFN-gamma or IFN-alpha. LIF had no effect on C4 levels in this system. Estrogen was found to down-modulate the induction of expression due to IFN-gamma. These data provide evidence for cytokine regulation of C4 expression in the synovium and for estrogen modulation of those effects.
为了确定滑膜中早期补体成分（C3和C4）表达的细胞因子诱导性，将外植体组织培养7天。通过特异性酶联免疫吸附测定法测量C3和C4的产生，并通过半定量PCR评估RNA。评估了白血病抑制因子（LIF）、γ干扰素（IFN-γ）、IFN-α和雌激素对C3和C4表达的影响。LIF、IFN-γ或IFN-α处理7天对C3水平无影响。相反，用IFN-γ或IFN-α处理7天的滑膜样本中C4水平显著诱导。LIF对该系统中的C4水平无影响。发现雌激素可下调IFN-γ诱导的表达。这些数据为滑膜中C4表达的细胞因子调节以及雌激素对这些作用的调节提供了证据。
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