Knapper J M, Morgan L M, Fletcher J M, Marks V
School of Biological Sciences, University of Surrey, Guildford, United Kingdom.
Horm Metab Res. 1995 Nov;27(11):485-90. doi: 10.1055/s-2007-980008.
Plasma concentrations of glucose dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1(7-36)amide (GLP-1[7-36]amide) were measured after milk ingestion in 15-18 day old piglets and after weaning diet ingestion in 33 day old piglets weaned at 21 days. Intestinal concentrations of these two hormones were also measured in unsuckled piglets of less than 24 h of age, and piglets whose ages corresponded with those used for plasma measurements. Suckling piglets showed a moderate glycaemic and insulinaemic response to milk ingestion. Plasma GIP and GLP-1(7-36)amide levels were significantly elevated at 1 and 3-h post-prandially. Weaned piglets showed a much more marked glucose and insulin response to meal ingestion. Plasma GIP and GLP-1(7-36)amide levels were again significantly elevated at 1 and 3 h in these animals. The mean plasma GIP response was greater in the weaned animals compared with the suckling animals at the time points investigated. The plasma GLP-1(7-36)amide response in contrast was significantly greater at 1 h in the suckling animals. In comparison, GIP concentrations in acid ethanol extracts of the small intestine were significantly higher during suckling and GLP-1(7-36)amide concentrations significantly higher after weaning. The circulating levels of both hormones seen during suckling and after weaning were far higher than those previously reported in humans. We conclude that both milk ingestion and the weaning diet are capable of stimulating GIP and GLP-1(7-36)amide in piglets and suggest that the levels of both hormones seen in this study may be important in adipose tissue metabolism at this time.
在15 - 18日龄仔猪摄入牛奶后以及在21日龄断奶的33日龄仔猪摄入断奶日粮后，测定了血浆中葡萄糖依赖性促胰岛素多肽（GIP）和胰高血糖素样肽 - 1（7 - 36）酰胺（GLP - 1[7 - 36]酰胺）的浓度。还测定了小于24小时龄的未哺乳仔猪以及与用于血浆测量的仔猪年龄相对应的仔猪小肠中这两种激素的浓度。哺乳仔猪对牛奶摄入表现出适度的血糖和胰岛素反应。餐后1小时和3小时血浆GIP和GLP - 1（7 - 36）酰胺水平显著升高。断奶仔猪对进食表现出更为明显的葡萄糖和胰岛素反应。在这些动物中，餐后1小时和3小时血浆GIP和GLP - 1（7 - 36）酰胺水平再次显著升高。在所研究的时间点，断奶动物的平均血浆GIP反应比哺乳动物更大。相比之下，哺乳动物在1小时时血浆GLP - 1（7 - 36）酰胺反应显著更大。相比之下，小肠酸性乙醇提取物中的GIP浓度在哺乳期间显著更高，而GLP - 1（7 - 36）酰胺浓度在断奶后显著更高。哺乳期间和断奶后观察到的这两种激素的循环水平远高于先前在人类中报道的水平。我们得出结论，摄入牛奶和断奶日粮都能够刺激仔猪体内的GIP和GLP - 1（7 - 36）酰胺，并表明本研究中观察到的这两种激素的水平此时可能在脂肪组织代谢中起重要作用。
