Nag A C, Sreepathi P, Lee M L, Reddan J R
Department of Biological Sciences, Oakland University, Rochester, Michigan 48309-4401, USA.
Cytobios. 1996;85(340):7-27.
The effect of hydrogen peroxide on cultured neonatal and adult cardiac myocytes was investigated. On neonatal cardiac myocytes the effect was very pronounced at a low concentration (0.03 mM), whereas the adult cardiac myocytes were resistant to the same concentration of H2O2. Dividing neonatal cardiac myocytes were more susceptible to H2O2 insult than the non-dividing adult cardiac myocytes. At a concentration of 0.1 mM H2O2, the neonatal cardiac myocytes were significantly damaged compared with the adult cardiac myocytes. Cardiac muscle cells from neonatal and adult hearts at high density culture were more tolerant to the oxidative insult by H2O2 than cells in low density culture. The damaging effect of H2O2 was very selective on F-actin in neonatal and adult cardiac muscle cells. The effect of H2O2 on myosin, titin, alpha-actinin, desmin or tubulin was not pronounced. Microscopical studies suggested a more marked protection by catalase than by glutathione reductase in the neonatal cells.
研究了过氧化氢对培养的新生和成年心肌细胞的影响。在新生心肌细胞上，低浓度（0.03 mM）时作用非常明显，而成年心肌细胞对相同浓度的H2O2具有抗性。正在分裂的新生心肌细胞比不分裂的成年心肌细胞更容易受到H2O2损伤。在0.1 mM H2O2浓度下，与成年心肌细胞相比，新生心肌细胞受到显著损伤。高密度培养的新生和成年心脏的心肌细胞比低密度培养的细胞对H2O2的氧化损伤更具耐受性。H2O2对新生和成年心肌细胞中的F-肌动蛋白的损伤作用非常具有选择性。H2O2对肌球蛋白、肌联蛋白、α-辅肌动蛋白、结蛋白或微管蛋白的作用不明显。显微镜研究表明，在新生细胞中，过氧化氢酶的保护作用比谷胱甘肽还原酶更显著。
