Drobnik J, Dabrowski R
Department of Pathophysiology, University School of Medicine, Lódź, Poland.
Cytobios. 1996;85(340):51-8.
The pineal gland hormone was shown to be involved in the regulation of repair and connective tissue accumulation in a wound. The hypothesis that melatonin injection could reverse the pinealectomy-induced elevation of the collagen content in scar tissue was verified. The effect of various melatonin doses (3, 30 and 100 micrograms/100 g body wt) on soluble, insoluble and total collagen content in the granulation tissue of a wound was investigated. The collagen level was estimated in pinealectomized rats, as well as in pinealectomized animals treated with melatonin. Ivalon sponges inserted subcutaneously were applied as a wound model. After 4 weeks the collagen content was measured as a hydroxyproline concentration in the dry tissue of the wound. The results showed that melatonin at the dose of 30 micrograms/100 g body wt significantly decreased both total and insoluble collagen content in the wounded tissue (p < 0.05), but other doses were ineffective. Pinealectomy increased the total (p = 0.05) and insoluble collagen level (p < 0.05) in the granulation tissue. Melatonin suppressed pinealectomy-induced elevation of the total and insoluble collagen content in the wounds (p < 0.05). No influence of the pineal gland on the soluble collagen content was observed. The results showed that melatonin was involved in the inhibitory control of the collagen content.
松果体激素被证明参与伤口修复和结缔组织积累的调节。褪黑素注射可逆转松果体切除引起的瘢痕组织中胶原蛋白含量升高这一假说得到了验证。研究了不同剂量褪黑素（3、30和100微克/100克体重）对伤口肉芽组织中可溶性、不可溶性和总胶原蛋白含量的影响。对松果体切除的大鼠以及用褪黑素治疗的松果体切除动物的胶原蛋白水平进行了评估。将皮下植入的Ivalon海绵用作伤口模型。4周后，将胶原蛋白含量作为伤口干组织中的羟脯氨酸浓度进行测量。结果表明，30微克/100克体重剂量的褪黑素显著降低了受伤组织中的总胶原蛋白和不可溶性胶原蛋白含量（p<0.05），但其他剂量无效。松果体切除增加了肉芽组织中的总胶原蛋白水平（p = 0.05）和不可溶性胶原蛋白水平（p<0.05）。褪黑素抑制了松果体切除引起的伤口中总胶原蛋白和不可溶性胶原蛋白含量的升高（p<0.05）。未观察到松果体对可溶性胶原蛋白含量的影响。结果表明，褪黑素参与了对胶原蛋白含量的抑制性控制。
