Takahashi H, Sasakura N, Noji M, Saito K
Faculty of Pharmaceutical Sciences, Laboratory of Molecular Biology and Biotechnology in Research Center of Medicinal Resources, Chiba University, Japan.
FEBS Lett. 1996 Aug 26;392(2):95-9. doi: 10.1016/0014-5793(96)00787-9.
A cDNA encoding a sulfate transporter was isolated from Arabidopsis thaliana. The isolated clone contained an open reading frame encoding a polypeptide of 658 amino acids, exhibiting the highest similarity (62%) with the sequence of the low-affinity sulfate transporter of a tropical legume Stylosanthes hamata. Northern blot analysis indicated the constitutive accumulation of a 2.6 kb length transcript in leaves and roots of seedlings. We also propose that A. thaliana contains three sulfate transporter genes which are expressed as 3.0, 2.7 and 2.6 kb length transcripts, respectively, in an organ-specific manner.
从拟南芥中分离出一个编码硫酸盐转运蛋白的cDNA。分离得到的克隆含有一个开放阅读框，编码一个由658个氨基酸组成的多肽，与热带豆科植物链荚豆的低亲和力硫酸盐转运蛋白序列具有最高的相似性（62%）。Northern杂交分析表明，在幼苗的叶片和根中组成性积累一种长度为2.6kb的转录本。我们还提出，拟南芥含有三个硫酸盐转运蛋白基因，它们分别以器官特异性方式表达为长度为3.0、2.7和2.6kb的转录本。
