Malygin A, Karpova G, Westermann P
Novosibirsk Institute of Bio-organic Chemistry, Siberian Division of Russian Academy of Sciences, Russia.
FEBS Lett. 1996 Aug 26;392(2):114-6. doi: 10.1016/0014-5793(96)00798-3.
Hybridization of two oligodeoxyribonucleotides (ON1 and ON2), complementary to opposite strands of the apical domain of Escherichia coli 4.5S RNA, was studied. ON1, complementary to bases 58-71, was not able to form a stable RNA-DNA hybrid whereas ON2, complementary to bases 38-53, was. Addition of both oligonucleotides at the same time resulted in the formation of a ternary complex permitting hybridization of ON1 and increasing hybridization of ON2. Under this condition, binary complexes of ON1 or ON2 with 4.5S RNA were not observed. The data demonstrate that hybridization of oligonucleotides to both strands of an RNA hairpin structure increases the efficiency of hybridization of either oligonucleotide.
研究了与大肠杆菌4.5S RNA顶端结构域相反链互补的两种寡脱氧核糖核苷酸（ON1和ON2）的杂交情况。与碱基58 - 71互补的ON1不能形成稳定的RNA - DNA杂交体，而与碱基38 - 53互补的ON2则可以。同时添加两种寡核苷酸会导致形成三元复合物，从而使ON1能够杂交，并增加ON2的杂交效率。在这种条件下，未观察到ON1或ON2与4.5S RNA的二元复合物。数据表明，寡核苷酸与RNA发夹结构的两条链杂交可提高任一寡核苷酸的杂交效率。
