Halachmi D, Eilam Y
Department of Bacteriology, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
FEBS Lett. 1996 Aug 26;392(2):194-200. doi: 10.1016/0014-5793(96)00799-5.
The Ca2+ -ATPase homolog of Saccharomyces cerevisiae, PMR1, cloned by Rudolph et al. (Cell 58 (1989) 133-145) is required for normal Golgi functions. We have investigated the role of Pmr1-protein in maintaining homeostasis of cytosolic free Ca2+ concentration ([Ca2+]i). It was found that exposure to moderately high Ca2+ concentrations led to elevated levels of [Ca2+]i in cells of pmr1 null mutant, in comparison with cells of pmr2 isogenic mutant (defective in cell-membrane Na+ - ATPase) and of an isogenic wild type. In addition, we showed that PMR1 deletion causes massive accumulation of Ca2+ in the vacuoles and affects the rates of Ca2+ influx and efflux.
酿酒酵母的Ca2 + -ATP酶同源物PMR1，由鲁道夫等人克隆（《细胞》58卷（1989年）133 - 145页），是正常高尔基体功能所必需的。我们研究了Pmr1蛋白在维持细胞质游离Ca2 +浓度（[Ca2 + ]i）稳态中的作用。结果发现，与pmr2同基因突变体（细胞膜Na + -ATP酶有缺陷）细胞和同基因野生型细胞相比，暴露于适度高Ca2 +浓度会导致pmr1缺失突变体细胞中的[Ca2 + ]i水平升高。此外，我们还表明，PMR1缺失会导致液泡中Ca2 +大量积累，并影响Ca2 +的流入和流出速率。
