Sagartz J E, Ozaki A, Capen C C
Department of Veterinary Biosciences, Ohio State University, Columbus, 43210, USA.
Toxicol Pathol. 1995 Nov-Dec;23(6):635-43. doi: 10.1177/019262339502300601.
The ability of FRTL-5 rat thyroid follicular cells to engulf latex beads by phagocytosis was evaluated using flow cytometry and compared to iodide trapping in response to selected growth factors, second messengers, and chemicals. Cell suspensions were analyzed to determine the percentage of fluorescence-positive cells as well as the fluorescence intensity of positive cells. Phagocytosis was stimulated by forskolin, cholera toxin, 8-Br-cAMP, calcitriol, and transforming growth factor-beta. In contrast, phagocytosis was inhibited by insulin, calcium, and aminotriazole, but not by sodium iodide. The results of this study showed that phagocytosis of latex beads was regulated in a manner similar to iodide trapping and could be altered by the addition of numerous compounds. Phagocytic activity was stimulated by both cAMP-dependent and cAMP-independent pathways. Flow cytometric evaluation of phagocytosis of fluorescent latex beads represents a simple, rapid, nonradioactive index of thyroid function in vitro.
利用流式细胞术评估了FRTL-5大鼠甲状腺滤泡细胞通过吞噬作用吞噬乳胶珠的能力，并将其与对选定生长因子、第二信使和化学物质的碘摄取进行了比较。分析细胞悬液以确定荧光阳性细胞的百分比以及阳性细胞的荧光强度。福斯可林、霍乱毒素、8-溴环磷酸腺苷、骨化三醇和转化生长因子-β可刺激吞噬作用。相反，胰岛素、钙和氨基三唑可抑制吞噬作用，但碘化钠不会。本研究结果表明，乳胶珠的吞噬作用与碘摄取的调节方式相似，并且可通过添加多种化合物而改变。吞噬活性受cAMP依赖性和cAMP非依赖性途径的刺激。荧光乳胶珠吞噬作用的流式细胞术评估代表了一种简单、快速、非放射性的体外甲状腺功能指标。
