Rabenstein K, McShane A J, McKenna M J, Dempsey E, Keaveny T V, Freaney R
Education and Research Centre, St. Vincent's Hospital, Dublin, Ireland.
Technol Health Care. 1996 Apr;4(1):67-76.
Direct sensor implantation for continuous biochemical monitoring has proved disappointing, but microdialysis sampling devices can serve as a biocompatible patient-sensor interface. We propose a novel "MiniShunt" extracorporeal microdialysis sampling circuit designed to sample core blood, and report the performance of different microdialysis probe membrane types and sizes with pumped and non-pumped blood flow in anaesthetised dogs. Discrete microdialysate and plasma samples collected during glucose and lactate monitoring were analysed with a YSI analyzer and showed microdialysis efficiency (% relative recovery) in excess of 95% for both analytes using fibres of greater than 70 mm2 internal surface area. Pumped veno-venous extracorporeal blood microdialysis sampling of this type could provide a suitable interface for future multi-analyte on-line biosensor applications in critically ill patients.
直接植入传感器进行连续生化监测的效果并不理想，但微透析采样装置可作为生物相容性良好的患者-传感器接口。我们提出了一种新型的“微型分流器”体外微透析采样回路，旨在采集核心血液，并报告在麻醉犬中使用泵注和非泵注血流时不同微透析探针膜类型和尺寸的性能。在葡萄糖和乳酸监测期间收集的离散微透析液和血浆样本，用YSI分析仪进行分析，结果表明，使用内表面积大于70 mm²的纤维时，两种分析物的微透析效率（相对回收率%）均超过95%。这种类型的泵注静脉-静脉体外血液微透析采样可为未来危重症患者的多分析物在线生物传感器应用提供合适的接口。
