Miller D J, Njenga M K, Parisi J E, Rodriguez M
Department of Immunology, Mayo Clinic and Foundation, Rochester, Minnesota, USA.
J Histochem Cytochem. 1996 Sep;44(9):1005-11. doi: 10.1177/44.9.8773566.
A contemporary view of autoimmunity suggests that self-reactivity is a normal phenomenon, in contrast to the classical association between autoimmunity and immunopathology. We have previously demonstrated that monoclonal antibody SCH94.03, a natural autoantibody with polyreactivity towards several purified protein and hapten antigens, promotes central nervous system remyelination when passively transferred to SJL/J mice chronically infected with Theiler's murine encephalomyelitis virus, an established experimental model of multiple sclerosis. In this study we characterized the autoreactivity of SCH94.03 with endogenous mouse tissue using immunoperoxide and multiple-color immunofluorescence staining techniques on frozen tissue sections. Within the nervous system, SCH94.03 labeled fibrous astrocytes, ependymal cells, ganglion satellite cells, and a sub-population of microglia, oligodendrocytes, and peripheral nervous system neurons. Outside the nervous system, SCH94.03 labeled gastrointestinal tract smooth muscle and luminal epithelium, erythrocytes, and interdigitating dendritic cells in peripheral lymphoid organs. These data indicate that SCH94.03 is a multi-organ reactive autoantibody and support the hypothesis that autoantibodies can have a beneficial rather than a pathogenic function in central nervous system demyelinating diseases.
与自身免疫和免疫病理学之间的经典关联不同，当代对自身免疫的观点认为自身反应性是一种正常现象。我们之前已经证明，单克隆抗体SCH94.03是一种对多种纯化蛋白和半抗原具有多反应性的天然自身抗体，当将其被动转移到慢性感染泰勒氏鼠脑脊髓炎病毒（一种既定的多发性硬化症实验模型）的SJL/J小鼠体内时，可促进中枢神经系统的髓鞘再生。在本研究中，我们使用免疫过氧化物和多色免疫荧光染色技术，在冷冻组织切片上对SCH94.03与内源性小鼠组织的自身反应性进行了表征。在神经系统内，SCH94.03标记了纤维状星形胶质细胞、室管膜细胞、神经节卫星细胞以及小胶质细胞、少突胶质细胞和外周神经系统神经元的一个亚群。在神经系统外，SCH94.03标记了胃肠道平滑肌和腔上皮、红细胞以及外周淋巴器官中的交错突细胞。这些数据表明SCH94.03是一种多器官反应性自身抗体，并支持自身抗体在中枢神经系统脱髓鞘疾病中可能具有有益而非致病功能的假说。
