脂质过氧化后蛋白质在线粒体亚颗粒中的分布。
Protein distribution is submitochondrial particles after lipid peroxidation.
作者信息
Eugui J, Gil C, Gómez J C, Segovia J
出版信息
Rev Esp Fisiol. 1977 Jun;33(2):129-33.
PMID:877380
Abstract
Three fractions of submitochondrial particles were isolated by differential centrifugation of mitochondrial inner membranes incubated with 1 mM ascorbate. Proteins were separated by polyacrylamide gel electrophoresis and their pattern was found to be different for the three fractions. These results are in agreement with previous work which showed the dissimilar enzyme distribution and chemical composition of these submitochondrial particles and pointed out the heterogeneity of the inner mitochondrial membrane.
摘要
通过对用1 mM抗坏血酸孵育的线粒体内膜进行差速离心，分离出了三个亚线粒体颗粒组分。蛋白质通过聚丙烯酰胺凝胶电泳进行分离，发现这三个组分的蛋白质图谱不同。这些结果与之前的研究一致，之前的研究表明这些亚线粒体颗粒的酶分布和化学组成不同，并指出线粒体内膜具有异质性。
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