Miyachi Y, Jokei K, Oka M, Hayashi T
Ajinomoto Co., Inc., Tokyo, Japan.
J Biomater Sci Polym Ed. 1996;7(9):805-16. doi: 10.1163/156856296x00147.
Two component random copolypeptide hydrogels consisting of N-hydroxyalkyl L-glutamine and L-leucine were prepared by carrying out aminolysis reactions with 3-amino-1-propanol(P) together with cross-linking reactors with 1,8-octamethylenediamine (OMDA) on hydrogels of the starting copolymers consisting of gamma-methyl-L-glutamate(M) and L-leucine(L). The relation between their bulk structure and properties was investigated with regard to the swelling ration in water, aqueous vapor permeability, tensile properties, and enzymatic degradation behavior in a pseudoextracellular fluid (PECF). The tensile property of the hydrogels was highly dependent on the swelling ratio in PECF, and on the hydrophobicity of the side chains, whose behavior was typical of an elastomer. It was shown that a common relation was obtained between the rate of water vapor permeabilities and the swelling ratio of hydrogels in PECF regardless of the difference of the nature of side chains. Biodegradation of the hydrogels in vitro by bromelain indicated that the degradation took place in bulk rather than on surface, and that the rate of degradation was also highly dependent on the swelling ratio of samples as well as on the hydrophobicity of the side chains of samples.
通过在由γ-甲基-L-谷氨酸（M）和L-亮氨酸（L）组成的起始共聚物水凝胶上，用3-氨基-1-丙醇（P）进行氨解反应，并与1,8-辛二胺（OMDA）进行交联反应，制备了由N-羟烷基-L-谷氨酰胺和L-亮氨酸组成的双组分随机共聚多肽水凝胶。研究了它们的本体结构与性能之间的关系，涉及在水中的溶胀率、水蒸气透过率、拉伸性能以及在伪细胞外液（PECF）中的酶降解行为。水凝胶的拉伸性能高度依赖于在PECF中的溶胀率以及侧链的疏水性，其行为是典型的弹性体。结果表明，无论侧链性质的差异如何，水蒸气透过率与水凝胶在PECF中的溶胀率之间都存在共同关系。菠萝蛋白酶在体外对水凝胶的生物降解表明，降解发生在本体而非表面，并且降解速率也高度依赖于样品的溶胀率以及样品侧链的疏水性。
