Taniguchi T, Haque M S, Sugiyama K, Okada K, Nakai Y, Kitazawa Y
Department of Ophthalmology, Gifu University School of Medicine, Japan.
J Ocul Pharmacol Ther. 1996 Summer;12(2):123-30. doi: 10.1089/jop.1996.12.123.
This study attempted to clarify the role of endothelin A and B (ETA and ETB) receptors in modulating aqueous humor dynamics in the rabbit eye. Intraocular pressure (IOP), aqueous flow, total outflow facility, and uveoscleral outflow were measured before, and 24 hours after, intravitreal injection (20 microliters) of a selective ETB agonist, sarafotoxin S6c (SRTX S6c, 10(-5) M), into one eye and its vehicle into the contralateral eye. The measurements were also performed before and after injection of a selective ETA antagonist, 97-139 (10(-2) M) + ET-1 (10(-5) M) into one eye, and vehicle + ET-1 (10(-5) M) into the contralateral eye. In the SRTX S6c-treated eye, total outflow facility increased significantly by 106% compared with that in the vehicle-treated eye 24 hours after injection; aqueous flow and uveoscleral outflow failed to change significantly. The ETA antagonist 97-139 inhibited the decrease in aqueous flow partially, but significantly, but failed to inhibit the increase in total outflow facility caused by ET-1. Therefore, both ETA and ETB play a significant role in modulating aqueous humor dynamics in the rabbit eye. ETA can modulate aqueous humor production, at least in part. ETB can modulate total outflow facility, and the possibility for ETB in modulating aqueous humor formation cannot be ruled out.
本研究试图阐明内皮素A和B（ETA和ETB）受体在调节兔眼房水动力学中的作用。向一只眼玻璃体内注射（20微升）选择性ETB激动剂沙拉毒素S6c（SRTX S6c，10⁻⁵ M），向对侧眼注射其溶媒，在注射前及注射后24小时测量眼压（IOP）、房水流量、总流出易度和葡萄膜巩膜流出。还向一只眼注射选择性ETA拮抗剂97 - 139（10⁻² M）+内皮素 - 1（ET - 1，10⁻⁵ M），向对侧眼注射溶媒+ET - 1（10⁻⁵ M），在注射前后进行测量。在接受SRTX S6c治疗的眼中，注射后24小时总流出易度与接受溶媒治疗的眼相比显著增加了106%；房水流量和葡萄膜巩膜流出未发生显著变化。ETA拮抗剂97 - 139部分但显著地抑制了房水流量的减少，但未能抑制ET - 1引起的总流出易度增加。因此，ETA和ETB在调节兔眼房水动力学中均起重要作用。ETA至少部分地可以调节房水生成。ETB可以调节总流出易度，并且不能排除ETB在调节房水形成方面的可能性。
