Savino E A, Varela A, Carregal M
Cátedra de Fisiología, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
Rev Esp Fisiol. 1996 Mar;52(1):9-13.
The rat tail artery during a 180 min incubation period in a medium containing glucose plus oxfenicine (an inhibitor of fatty acid oxidation) did not show changes in the contractile responses to adrenaline. In a substrate-free medium the extent of the contractions underwent a slight decrease during the last 60 min of incubation. When the substrate-free medium contained 2-deoxyglucose (an inhibitor of glycolysis and glycogenolysis) or oxfenicine, the decline of the contractile activity developed faster and attained a similar extent with each inhibitor. When the substrate-free medium contained 2-deoxyglucose together with oxfenicine or methylpalmoxirate (an inhibitor of fatty acid oxidation) the arteries displayed a pronounced and early fall in the contraction strength. These data suggest that in the presence of glucose the reserve substrates are not necessary as fuel source for the arterial contractions. However, in substrate-free conditions they constitute an important energy source. Furthermore, glycogen and triacylglycerol share the supply of energy and there does not seem to be any other reserve material in the smooth muscle of the rat tail artery.
在含有葡萄糖加奥芬尼辛（一种脂肪酸氧化抑制剂）的培养基中孵育180分钟期间，大鼠尾动脉对肾上腺素的收缩反应未显示出变化。在无底物培养基中，孵育最后60分钟期间收缩程度略有下降。当无底物培养基含有2-脱氧葡萄糖（一种糖酵解和糖原分解抑制剂）或奥芬尼辛时，收缩活性的下降发展得更快，并且每种抑制剂达到的程度相似。当无底物培养基含有2-脱氧葡萄糖与奥芬尼辛或甲基棕榈酰肉碱（一种脂肪酸氧化抑制剂）时，动脉显示出收缩强度明显且早期下降。这些数据表明，在有葡萄糖存在的情况下，储备底物并非动脉收缩的必要燃料来源。然而，在无底物条件下，它们构成重要的能量来源。此外，糖原和三酰甘油共同提供能量，并且在大鼠尾动脉平滑肌中似乎没有任何其他储备物质。
