阿尔茨海默病患者生前腰椎脑脊液、血清及死后脑室中免疫反应性载脂蛋白E的情况
Intra vitam lumbar cerebrospinal fluid and serum and postmortem ventricular immunoreactive apolipoprotein E in patients with Alzheimer's disease.
作者信息
Rösler N, Wichart I, Jellinger K A
机构信息
Ludwig Boltzmann Institute of Clinical Neurobiology, Lainz Hospital, Vienna, Austria.
出版信息
J Neurol Neurosurg Psychiatry. 1996 Apr;60(4):452-4. doi: 10.1136/jnnp.60.4.452.
Using a specific enzyme linked immunosorbent assay (ELISA) method, total apolipoprotein E immunoreactivity (tApoE-IR) was measured in premortem lumbar CSF and serum of patients with "probable" Alzheimer's disease and in postmortem ventricular CSF of patients with Alzheimer's disease confirmed by necropsy. Concentrations were compared with those from patients with other neurological diseases and controls. The mean serum:lumbar CSF ratio of ApoE-IR was 15.9 suggesting that the main portion of lumbar ApoE-IR is synthesised intrathecally. No significant differences in ApoE-IR between patients with Alzheimer's disease and the other groups were detected in either CSF compartment. In lumbar CSF, there was no correlation between ApoE-IR of patients with Alzheimer's disease and their mini mental state scores. These results suggest that the diagnostic value of ApoE-IR measurements in CSF of patients with Alzheimer's disease as a single determination is less than that of other markers, in particular tau protein. On the other hand, ApoE determinations could be useful as part of a neurochemical profile of Alzheimer's disease.
采用特定的酶联免疫吸附测定（ELISA）方法，对“可能”患有阿尔茨海默病患者生前的腰椎脑脊液和血清以及经尸检确诊为阿尔茨海默病患者死后的脑室脑脊液中的总载脂蛋白E免疫反应性（tApoE-IR）进行了测量。将这些浓度与患有其他神经系统疾病的患者及对照组的浓度进行了比较。ApoE-IR的血清与腰椎脑脊液平均比值为15.9，表明腰椎ApoE-IR的主要部分是在鞘内合成的。在任何一个脑脊液腔室中，均未检测到阿尔茨海默病患者与其他组之间ApoE-IR存在显著差异。在腰椎脑脊液中，阿尔茨海默病患者的ApoE-IR与其简易精神状态评分之间无相关性。这些结果表明，作为单一测定指标，ApoE-IR测量在阿尔茨海默病患者脑脊液中的诊断价值低于其他标志物，尤其是tau蛋白。另一方面，ApoE测定作为阿尔茨海默病神经化学特征的一部分可能会有用。
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