Baggesen D L, Wegener H C, Christensen J P
Danish Veterinary Laboratory, Copenhagen, Denmark.
APMIS. 1996 Jun;104(6):411-8.
During the summer of 1993 an outbreak of human salmonellosis caused by Salmonella serovar Saintpaul occurred in Denmark. A total of 35 isolates originating from pigs, turkeys and imported foodstuffs, and 10 human isolates were compared following their characterization by agglutination of the 0:5 factor, antibiogram typing, plasmid profiling, ribotyping and pulsed field gel electrophoresis, in order to identify the most probable source of infection. After typing, the source of the investigated outbreak remains obscure because so far no isolates with traits of the outbreak strain have been recovered from production animals. Presence of the 0:5 factor and absence of plasmids in human and porcine isolates pointed to pork as the source of infection, whereas human isolates and all Danish isolates from turkeys had the same ribotype, indicating that turkey was the infection source. A possible explanation for the failure to find isolates with traits of the outbreak strain could be the presence of a third, but so far unidentified, source. The present investigation illustrates the necessity of using more than one epidemiological typing method for outbreak investigation. This is especially important when the organism involved is relatively uncommon and little is known about its diversity and distribution.
1993年夏天，丹麦发生了由圣保罗沙门氏菌引起的人类沙门氏菌病疫情。对35株分别源自猪、火鸡和进口食品的分离株以及10株人类分离株，通过0:5因子凝集、抗菌谱分型、质粒图谱分析、核糖体分型和脉冲场凝胶电泳进行鉴定，以确定最可能的感染源。分型后，此次调查疫情的源头仍不明确，因为到目前为止，尚未从生产动物中分离出具有疫情菌株特征的菌株。人类和猪的分离株中存在0:5因子且无质粒，这表明猪肉是感染源，而人类分离株和丹麦所有火鸡分离株具有相同的核糖体分型，表明火鸡是感染源。未能找到具有疫情菌株特征的分离株的一个可能解释是存在第三种但迄今尚未确定的来源。本调查说明了在疫情调查中使用多种流行病学分型方法的必要性。当所涉及的微生物相对不常见且对其多样性和分布了解甚少时，这一点尤为重要。
