Andrejeva J, Merits A, Rabenstein F, Puurand U, Järvekülg L
Institute of Chemical Physics and Biophysics, Tallinn, Estonia.
Arch Virol. 1996;141(7):1207-19. doi: 10.1007/BF01718825.
The sequences of the 3'-terminal 1145 nucleotides of two non-aphid transmissible (NAT) isolates (Ali and Juliniere) and one aphid transmissible (AT) isolate (Rouge) of potato virus A (PVA) RNA were determined. Those sequences contained the complete coding region of the coat protein (CP) followed by a 3'-nontranslated region (3'-NTR) of 225 (Ali and Juliniere) and 227 (Rouge) nucleotides. The obtained sequences were compared to those of the 3'-regions of four published PVA isolates and a virus described as tamarillo mosaic virus (TamMV) which on the basis of sequence data is a strain of PVA. The analysis of the 3'-terminal region of PVA isolates indicated that the CP N-terminal variable domain (32 residues) divides PVA isolates into two subgroups, where only tripeptide DAG correlates with aphid transmissibility. In addition to DAG/DAS sequence we found four other amino acids at the N-terminus of PVA CP, which are conserved in two subgroups. The central region (core part and C-termini) of CP is highly conserved among all PVA isolates (96.6 to 99.6%). 3'-NTR, separates PVA-isolates into two subgroups on the basis of its length and homology.
测定了马铃薯A病毒（PVA）RNA的两个非蚜传（NAT）分离株（Ali和Juliniere）以及一个蚜传（AT）分离株（Rouge）3'-末端1145个核苷酸的序列。这些序列包含衣壳蛋白（CP）的完整编码区，随后是225个核苷酸（Ali和Juliniere）以及227个核苷酸（Rouge）的3'-非翻译区（3'-NTR）。将获得的序列与四个已发表的PVA分离株以及一种被描述为番茄环斑病毒（TamMV）的病毒的3'-区域序列进行了比较，基于序列数据，TamMV是PVA的一个株系。对PVA分离株3'-末端区域的分析表明，CP的N端可变结构域（32个残基）将PVA分离株分为两个亚组，其中只有三肽DAG与蚜传性相关。除了DAG/DAS序列外，我们在PVA CP的N端还发现了另外四个氨基酸，它们在两个亚组中是保守的。CP的中央区域（核心部分和C末端）在所有PVA分离株中高度保守（96.6%至99.6%）。3'-NTR根据其长度和同源性将PVA分离株分为两个亚组。
