猪腺病毒五聚体基座基因的基因组定位和核苷酸序列

Genomic location and nucleotide sequence of a porcine adenovirus penton base gene.

作者信息

McCoy R J, Johnson M A, Studdert M J, Sheppard M

机构信息

Commonwealth Scientific and Industrial Research Organisation, Division of Animal Research, Parkville, Victoria, Australia.

出版信息

Arch Virol. 1996;141(7):1367-75. doi: 10.1007/BF01718838.

DOI:10.1007/BF01718838

PMID:8774695

Abstract

The putative penton base gene of a porcine adenovirus serotype 3 (PAV3) has been identified, cloned and sequenced. The genomic location of the PAV3 penton base was deduced by probing a Southern blot with a polymerase chain reaction generated product containing the human adenovirus type 2 (HAV2) penton base gene. Sequencing revealed an open reading frame (ORF) of 1527 nucleotides coding for a polypeptide of 509 amino acids. However, cDNA analysis indicated an acceptor splice site one nucleotide upstream of the second ATG in the ORF. This produced an ORF of 1452 nucleotides coding for a polypeptide of 484 amino acids with a calculated molecular weight of 54.5 kDa. Comparison with the HAV2 penton base amino acid sequence revealed the putative PAV3 penton base homologue to be 87 amino acids shorter with an overall amino acid homology of approximately 65%. Comparison with the penton base proteins of other HAV types revealed a region between amino acid positions 283 and 379 with no similarity.

摘要

猪腺病毒3型（PAV3）假定的五邻体基底基因已被鉴定、克隆和测序。通过用含有人腺病毒2型（HAV2）五邻体基底基因的聚合酶链反应产物探测Southern印迹，推断出PAV3五邻体基底的基因组位置。测序显示一个1527个核苷酸的开放阅读框（ORF），编码一个509个氨基酸的多肽。然而，cDNA分析表明在该ORF中第二个ATG上游一个核苷酸处有一个受体剪接位点。这产生了一个1452个核苷酸的ORF，编码一个484个氨基酸的多肽，计算分子量为54.5 kDa。与HAV2五邻体基底氨基酸序列比较显示，假定的PAV3五邻体基底同源物短87个氨基酸，总体氨基酸同源性约为65%。与其他HAV类型的五邻体基底蛋白比较显示，在氨基酸位置283和379之间没有相似性区域。