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A rapid method for detecting specific amplified PCR fragments in microtiter plates.一种在微孔板中检测特异性扩增PCR片段的快速方法。
Nucleic Acids Res. 1996 Aug 15;24(16):3280-1. doi: 10.1093/nar/24.16.3280.
2
A highly sensitive and fast nonradioactive method for detection of polymerase chain reaction products.
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One armed PCR (OA-PCR): amplification of genomic DNA from a single primer domain.单引物PCR（OA-PCR）：从单个引物结构域扩增基因组DNA。
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Rapid isolation of flanking genomic DNA using biotin-RAGE, a variation of single-sided polymerase chain reaction.使用生物素-RAGE（一种单侧聚合酶链反应变体）快速分离侧翼基因组DNA。
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Systematic characterization of 2'-deoxynucleoside- 5'-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA.通过聚合酶链反应对2'-脱氧核苷-5'-三磷酸类似物作为DNA聚合酶底物进行系统表征，并对修饰DNA的酶促生产进行动力学研究。
Nucleic Acids Res. 2006;34(19):5383-94. doi: 10.1093/nar/gkl637. Epub 2006 Sep 29.

本文引用的文献

1
A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral pathogens.
J Virol Methods. 1993 Dec 15;45(2):201-18. doi: 10.1016/0166-0934(93)90104-y.
2
Quantification of polymerase chain reaction products by affinity-based hybrid collection.基于亲和的杂交捕获法对聚合酶链反应产物进行定量分析。
Nucleic Acids Res. 1988 Dec 9;16(23):11327-38. doi: 10.1093/nar/16.23.11327.
3
Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes.用固定化的序列特异性寡核苷酸探针进行扩增DNA的遗传分析。
Proc Natl Acad Sci U S A. 1989 Aug;86(16):6230-4. doi: 10.1073/pnas.86.16.6230.
4
Microplate hybridization of amplified viral DNA segment.扩增病毒DNA片段的微孔板杂交
J Clin Microbiol. 1990 Jun;28(6):1469-72. doi: 10.1128/jcm.28.6.1469-1472.1990.
5
Quantitative analysis of polymerase chain reaction (PCR) products using primers labeled with biotin and a fluorescent dye.使用生物素和荧光染料标记的引物对聚合酶链反应（PCR）产物进行定量分析。
Anal Biochem. 1991 Mar 2;193(2):231-5. doi: 10.1016/0003-2697(91)90014-k.
6
Direct analysis of polymerase chain reaction products using enzyme-linked immunosorbent assay techniques.
Anal Biochem. 1991 Oct;198(1):86-91. doi: 10.1016/0003-2697(91)90510-z.

一种在微孔板中检测特异性扩增PCR片段的快速方法。

A rapid method for detecting specific amplified PCR fragments in microtiter plates.

作者信息

Ortiz A, Ritter E

机构信息

CIMA-Granja Modelo, Vitoria-Gasteiz, Spain.

出版信息

Nucleic Acids Res. 1996 Aug 15;24(16):3280-1. doi: 10.1093/nar/24.16.3280.

DOI:10.1093/nar/24.16.3280
PMID:8774915
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC146076/
Abstract

A simple method is presented to circumvent laborious and time consuming electrophoretic separations of specific PCR amplification products. Specific target DNA is amplified using nucleotides labelled with DIG-dUTP or biotin-dCTP. The labelled PCR products are separated from unincorporated nucleotides or oligonucleotides by using a positively charged DEAE cellulose matrix. Amplification products are visualized directly in the matrix using immunoenzymatic methods or streptavidin-conjugated enzymes. The detection process can be carried out within 2 h, allows the processing of large sample sizes and can potentially be automated.

摘要

本文提出了一种简单的方法，以规避对特定PCR扩增产物进行费力且耗时的电泳分离。使用用DIG-dUTP或生物素-dCTP标记的核苷酸扩增特定的目标DNA。通过使用带正电荷的DEAE纤维素基质，将标记的PCR产物与未掺入的核苷酸或寡核苷酸分离。使用免疫酶法或链霉亲和素偶联酶直接在基质中观察扩增产物。检测过程可在2小时内完成，允许处理大量样本，并且有可能实现自动化。