Kozłowski W, Kulig A, Czkwianianc E, Bak-Romaniszyn L, Płaneta-Małecka I, Przybylska B
Department of Diagnostic, Military Medical Academy of Lódź.
Rocz Akad Med Bialymst. 1995;40(3):678-84.
The aim of this study was to investigate the consequence of Helicobacter pylori eradication on gastric mucosa and antral G and D-cells. Forty children, aged 5-17 years with Helicobacter pylori infection were assessed. Helicobacter pylori was detected by a urease test and identified by serological and microbiological methods. Twenty children were again assessed after the therapy (the combination of colloid bismuth subcitrate, amoxycillin and metronidazole). Gastroscopic examination was performed and at least six bioptic specimens were taken from the antrum, body and fundus. Tissue samples, processed with the paraffin method and stained with hematoxyllin and eosin, were assessed. Monoclonal antiserum Gastrin PAP kit 516 and somatostatin PAP kit 512 (DAKO) in the peroxidase-antiperoxidase technique (PAP) have been used to detect G and D-cells. Helicobacter pylori in the gastric mucosa was demonstrated with the Giemsa method. The results show the coincidence of Helicobacter pylori infection and the count of antral G and D-cells and active chronic gastritis in children. After the treatment Helicobacter pylori was eradicated in 70% of children. In 34% of these cases the eradication was followed by a diminution of activity of gastric antral mucosa inflammation and in 20% of these children the resolution of the inflammatory infiltration in the gastric mucosa was seen. A decrease of the antral G and D-cells count and also a diminution of G/D index in these cases were observed.
本研究旨在探讨根除幽门螺杆菌对胃黏膜及胃窦G细胞和D细胞的影响。对40名年龄在5至17岁的幽门螺杆菌感染儿童进行了评估。通过尿素酶试验检测幽门螺杆菌，并采用血清学和微生物学方法进行鉴定。20名儿童在接受治疗（枸橼酸铋钾、阿莫西林和甲硝唑联合用药）后再次接受评估。进行了胃镜检查，并从胃窦、胃体和胃底至少采集了6份活检标本。对采用石蜡法处理并用苏木精和伊红染色的组织样本进行了评估。使用过氧化物酶抗过氧化物酶技术（PAP）中的胃泌素单克隆抗血清PAP试剂盒516和生长抑素PAP试剂盒512（达科公司）来检测G细胞和D细胞。采用吉姆萨法显示胃黏膜中的幽门螺杆菌。结果显示儿童幽门螺杆菌感染与胃窦G细胞和D细胞计数以及活动性慢性胃炎之间存在相关性。治疗后，70%的儿童幽门螺杆菌被根除。在这些病例中，34%的患儿根除幽门螺杆菌后胃窦黏膜炎症活动度降低，20%的患儿胃黏膜炎症浸润消退。观察到这些病例中胃窦G细胞和D细胞计数减少，G/D指数也降低。
