苯并[a]芘二醇环氧化物导致的DNA链断裂。
DNA strand scission by benzo[a]pyrene diol epoxides.
作者信息
Gamper H B, Tung A S, Straub K, Bartholomew J C, Calvin M
出版信息
Science. 1977 Aug 12;197(4304):671-4. doi: 10.1126/science.877583.
PMID:877583
Abstract
Syn-and anti-benzo[a]pyrene diol epoxides elicit a concentration-dependent nicking of superhelical Col E1 DNA in an in vitro reaction monitored by agarose gel electrophoresis and electron microscopy. This strand scission represents less than 1 percent of the DNA modification by diol epoxide. Kinetic analysis implicates the formation of unstable phosphotriesters, hydrolysis of which nick the DNA.
摘要
顺式和反式苯并[a]芘二氢二醇环氧化物在体外反应中会引发超螺旋Col E1 DNA浓度依赖性的切口，该反应通过琼脂糖凝胶电泳和电子显微镜进行监测。这种链断裂占二醇环氧化物对DNA修饰的不到1%。动力学分析表明形成了不稳定的磷酸三酯，其水解导致DNA切口。
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