Kedziora J, Buczyński A
Department of Physiology, Military Medical Academy, Lodz, Poland.
Int J Occup Med Environ Health. 1996;9(1):45-51.
Our investigations were carried out on blood platelets obtained from 52 males, aged 25-48, free from any systemic disease, hospitalized because of extremities inferioris fractures without clinical complications. The effect of immobilization on oxygen metabolism and function of human blood platelets was studied. Blood was taken from patients after 14, 28 and 90 days of bed rest and platelets were isolated by a differential centrifugation technique. The comparative control group consisted of 41 healthy individuals at their normal physical activity. Short and long term bed rest had different effect on estimated enzymes activities: SOD-1, GPx-SH, Ct (and lipid peroxidation indicators) (MDA-and TXA2). After 14 and 28 days, the enzyme activities significantly decreased, whereas MDA and TXA2 concentrations increased. It was also found that a 14 and 28-day immobilization increased the blood platelets aggregation. However, after 90 days the tendency towards normalization of all the estimated parameters was observed.
我们的研究是针对52名年龄在25至48岁之间、无任何系统性疾病、因下肢骨折住院且无临床并发症的男性的血小板进行的。研究了固定对人体血小板氧代谢和功能的影响。在卧床休息14天、28天和90天后采集患者血液，采用差速离心技术分离血小板。比较对照组由41名正常进行体育活动的健康个体组成。短期和长期卧床休息对估计的酶活性（超氧化物歧化酶-1、谷胱甘肽过氧化物酶-巯基、过氧化氢酶，以及脂质过氧化指标（丙二醛和血栓素A2））有不同影响。在14天和28天后，酶活性显著降低，而丙二醛和血栓素A2浓度升高。还发现，14天和28天的固定会增加血小板聚集。然而，90天后观察到所有估计参数有趋于正常化的趋势。
