• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Periplasmic expression of biologically active vesicular stomatitis virus phosphoprotein P in Escherichia coli.

作者信息

Pahari S, Pari A, Chattopadhyay D J

机构信息

Department of Biochemistry, University College of Science, Calcutta, India.

出版信息

Protein Expr Purif. 1996 Jun;7(4):384-8. doi: 10.1006/prep.1996.0057.

DOI:10.1006/prep.1996.0057
PMID:8776756
Abstract

Overexpression of a clone of vesicular stomatitis virus phosphoprotein P (New Jersey serotype) using T7 promoter with phoA leader sequence and a simpler two-step purification procedure of the expressed protein has been developed. The purified protein retains its ability to activate the transcription reaction. Comparative transcriptional assay using the protein P purified from periplasmic space and from cytosol (in the form of inclusion body) of Escherichia coli establishes the fact that the former is 10 times more efficient than the latter in activating the transcription reaction in vitro.

摘要

相似文献

1
Periplasmic expression of biologically active vesicular stomatitis virus phosphoprotein P in Escherichia coli.
Protein Expr Purif. 1996 Jun;7(4):384-8. doi: 10.1006/prep.1996.0057.
2
Cloning of the chandipura virus phosphoprotein encoding gene and its expression in Escherichia coli.
Cell Mol Biol Res. 1994;40(7-8):693-8.
3
Expression of the vesicular stomatitis virus nucleocapsid protein gene in Escherichia coli: analysis of its biological activity in vitro.水疱性口炎病毒核衣壳蛋白基因在大肠杆菌中的表达：其体外生物学活性分析
Virology. 1993 Mar;193(1):340-7. doi: 10.1006/viro.1993.1130.
4
Identification of a set of proteins (C' and C) encoded by the bicistronic P gene of the Indiana serotype of vesicular stomatitis virus and analysis of their effect on transcription by the viral RNA polymerase.鉴定水泡性口炎病毒印第安纳血清型双顺反子P基因编码的一组蛋白质（C'和C），并分析它们对病毒RNA聚合酶转录的影响。
Virology. 1996 Apr 15;218(2):335-42. doi: 10.1006/viro.1996.0202.
5
Cloning and expression of the phosphoprotein gene of Newcastle disease virus in Escherichia coli.
J Biochem Mol Biol Biophys. 2002 Apr;6(2):117-21. doi: 10.1080/10258140290027252.
6
Expression of functionally active cytochrome b5 in Escherichia coli: isolation, purification, and use of the immobilized recombinant heme protein for affinity chromatography of electron-transfer proteins.功能性活性细胞色素b5在大肠杆菌中的表达：固定化重组血红素蛋白的分离、纯化及其用于电子传递蛋白亲和色谱分析
Biochemistry (Mosc). 1997 Apr;62(4):401-11.
7
Expression, refolding, and characterization of human soluble BAFF synthesized in Escherichia coli.在大肠杆菌中合成的人可溶性B细胞活化因子的表达、复性及特性分析
Protein Expr Purif. 2005 May;41(1):199-206. doi: 10.1016/j.pep.2005.01.001.
8
High-level expression and one-step purification of recombinant dengue virus type 2 envelope domain III protein in Escherichia coli.重组登革2型病毒包膜结构域III蛋白在大肠杆菌中的高效表达及一步纯化
Protein Expr Purif. 2004 Jan;33(1):80-91. doi: 10.1016/j.pep.2003.09.009.
9
Optimization of BLyS production and purification from Escherichia coli.从大肠杆菌中优化B淋巴细胞刺激因子（BLyS）的生产与纯化
Protein Expr Purif. 2005 Feb;39(2):237-46. doi: 10.1016/j.pep.2004.10.006.
10
Expression and purification of anthrax toxin protective antigen from Escherichia coli.炭疽毒素保护性抗原在大肠杆菌中的表达与纯化
Protein Expr Purif. 1996 Feb;7(1):33-8. doi: 10.1006/prep.1996.0005.