Burd G, Ward O P
Department of Biology, University of Waterloo, ON, Canada.
Can J Microbiol. 1996 Aug;42(8):791-7. doi: 10.1139/m96-099.
A strain of Pseudomonas marginalis PD-14B, isolated from polycyclic aromatic hydrocarbon (PAH) contaminated soil, produced an extracellular surface-active factor of high molecular weight containing protein and lipopolysaccharide. A crude preparation of the factor, obtained from the culture both of cells grown in mineral salts (MS) medium with succinate and yeast extract, affected the extent of PAH degradation by the bacterial cells and prevented flocculation of PAHs in an aqueous suspension. A washed suspension of P. marginalis cells also prevented flocculation of PAH suspensions and emulsified liquid hydrocarbons. Incubation of the factor and the factor-producing strain in the presence of different PAHs resulted in gradual increase of turbidity of the PAH dispersions. The factor markedly stimulated rate and extent of turbidity generation by bacterial cells. The surface-active factor may promote infection of lettuce and other plants by this known pathogen and may also have application in bioremediation.
从多环芳烃（PAH）污染土壤中分离出的一株边缘假单胞菌PD - 14B产生了一种含有蛋白质和脂多糖的高分子量细胞外表面活性因子。该因子的粗制品是从在含有琥珀酸盐和酵母提取物的矿物盐（MS）培养基中生长的细胞培养物中获得的，它影响了细菌细胞对PAH的降解程度，并防止了PAHs在水悬浮液中的絮凝。边缘假单胞菌细胞的洗涤悬浮液也能防止PAH悬浮液的絮凝并乳化液态烃。在不同PAHs存在的情况下，将该因子与产生该因子的菌株一起培养，导致PAH分散液的浊度逐渐增加。该因子显著刺激了细菌细胞产生浊度的速率和程度。这种表面活性因子可能会促进这种已知病原体对生菜和其他植物的感染，并且在生物修复中也可能有应用。
