Siamblis D, Karnabatidis D, Hatjikondi O, Kalogeropoulou C, Kardamakis D, Dimopoulos J
University of Patras Medical School, Department of Radiology, Greece.
Eur J Radiol. 1996 Feb;21(3):220-4. doi: 10.1016/0720-048X(95)00714-2.
We use a modification of the digital substraction angiography (DSA) technique to visualize the vascular system of the chorioallantoic membrane (CAM) of the developing chick embryo. After catheterization of the central vein of the CAM with a metallic needle and injection of contrast medium, angiography was performed at specified follow-up intervals (1-2.5 images per second) using the Philips DVI-S Angiography Unit. By applying the vascular casting technique we were able to visualize the vessels of the CAM and to study the existing complex vascular-anatomical relationships. Our results show that DSA demonstrates the development of the circulatory system of the chick embryo (heart and vessels) and the vessels of its CAM effectively. By applying for the first time interventional radiology techniques on the chick embryo system, it has been possible to study morphological changes of the vessels during angiogenesis in vivo. These results suggest that this technique can be a proven and valuable tool in the quantitative assessment of angiogenesis.
我们采用数字减影血管造影（DSA）技术的一种改良方法来观察发育中的鸡胚绒毛尿囊膜（CAM）的血管系统。用金属针经导管插入CAM的中央静脉并注入造影剂后，使用飞利浦DVI - S血管造影设备，按照指定的随访间隔（每秒1 - 2.5幅图像）进行血管造影。通过应用血管铸型技术，我们能够观察到CAM的血管，并研究现有的复杂血管解剖关系。我们的结果表明，DSA能有效显示鸡胚循环系统（心脏和血管）及其CAM血管的发育情况。通过首次将介入放射学技术应用于鸡胚系统，得以在体内研究血管生成过程中血管的形态变化。这些结果表明，该技术可成为血管生成定量评估中一种经证实且有价值的工具。
