Bellin M E, Hawkins H E, Oyarzo J N, Vanderboom R J, Ax R L
Department of Animal Sciences, University of Arizona, Tucson 85721, USA.
J Anim Sci. 1996 Jan;74(1):173-82. doi: 10.2527/1996.741173x.
A monoclonal antibody (M1) was produced against seminal fluid heparin-binding proteins (HBP) from a vasectomized bull. In the first part of this study, the presence of HBP in sperm or seminal fluid was determined for 53 bulls with an ELISA using M1. Bulls (8 to 18 per pasture) were bred to 1,114 cows at ratios of 1 bull:25 cows. Bulls with detectable HBP on sperm membranes were 11 percentage points more fertile than bulls with undetectable HBP in sperm membranes. In the second part of this study, three sperm, membrane HBP approximately 30, 24, and 21.5 kDa were identified with Western blots using M1. Santa Gertrudis bulls (n = 64) were bred to 1,354 Santa Gertrudis cows in groups with 2 to 11 bulls. Bulls with those three HBP (Group A) or a single 30-kDa HBP (Group B) in sperm membranes had the greatest fertility, ranging from 74.4 to 89.9% (mean = 81.5%) of the palpated cows that were pregnant. Bulls with the 21.5- and 30-kDa HBP (i.e., the 24-kDa HBP was absent; Group C) had a reduced fertility of 61.3%. Bulls without detectable HBP (Group D) resulted in 41.9% of 186 cows palpated pregnant. Bulls in Groups A and B were more (P < .01) fertile than all other groups. In conclusion, the presence of HBP in sperm membranes was indicative of the fertility potential of bulls.
制备了一种针对输精管结扎公牛精液肝素结合蛋白（HBP）的单克隆抗体（M1）。在本研究的第一部分，使用M1通过酶联免疫吸附测定法（ELISA）对53头公牛的精子或精液中HBP的存在情况进行了检测。将公牛（每个牧场8至18头）以1头公牛比25头母牛的比例与1114头母牛配种。精子膜上可检测到HBP的公牛比精子膜上未检测到HBP的公牛繁殖力高11个百分点。在本研究的第二部分，使用M1通过蛋白质免疫印迹法鉴定出三种精子膜HBP，分子量约为30、24和21.5 kDa。将64头圣赫特鲁迪斯公牛分成每组2至11头的小组，与1354头圣赫特鲁迪斯母牛配种。精子膜上含有这三种HBP的公牛（A组）或仅含有一种30 kDa HBP的公牛（B组）繁殖力最强，经触诊怀孕的母牛比例在74.4%至89.9%之间（平均为81.5%）。含有21.5 kDa和30 kDa HBP（即不含24 kDa HBP；C组）的公牛繁殖力降低至61.3%。未检测到可检测HBP的公牛（D组）使186头经触诊的母牛中有41.9%怀孕。A组和B组的公牛比所有其他组的公牛繁殖力更强（P < 0.01）。总之，精子膜中HBP的存在表明了公牛的繁殖潜力。
