Tolbert W D, Moll J R, Bauerle R, Kretsinger R H
Department of Biology, University of Virginia, Charlottesville 22903, USA.
Proteins. 1996 Mar;24(3):407-8. doi: 10.1002/(SICI)1097-0134(199603)24:3<407::AID-PROT16>3.0.CO;2-Q.
3-Deoxy-D-manno-octulosonate-8-phosphate (KDOP) synthase catalyzes the production of KDOP from phosphoenolpyruvate (PEP) and arabinose-5-phosphate (A5P). In gram-negative bacteria KDOP is subsequently dephosphorylated, cytidylylated, and linked to lipid A and is required for lipid A incorporation into the outer membrane (Raetz, Annu. Rev. Biochem. 59:129-170, 1990). We have crystallized two forms of KDOP synthase belonging to space groups I23 or I2(1)3, one with a = b = c = 118.0 A and the other with a = b = c = 233 A.
3-脱氧-D-甘露-辛酮糖酸-8-磷酸（KDOP）合酶催化由磷酸烯醇丙酮酸（PEP）和阿拉伯糖-5-磷酸（A5P）生成KDOP。在革兰氏阴性菌中，KDOP随后会被去磷酸化、胞苷酰化，并与脂多糖A连接，这是脂多糖A整合到外膜所必需的（雷茨，《生物化学年度评论》59:129 - 170，1990年）。我们已经结晶出属于I23或I2(1)3空间群的两种形式的KDOP合酶，一种的a = b = c = 118.0埃，另一种的a = b = c = 233埃。
