Cho J Y, Nam S Y, Kim S G
College of Pharmacy, Duksung Women's University, Seoul, Korea.
Xenobiotica. 1995 Aug;25(8):791-8. doi: 10.3109/00498259509061894.
The effects of 1-benzylimidazole on the expression of microsomal epoxide hydrolase (mEH) gene were examined in rat. Immunoblot analyses showed that hepatic microsomes isolated from rat treated with 1-benzylimidazole at 25, 50 or 100 mg/kg/day for 3 or 6 days exhibited 2-3-fold increases in mEH levels, relative to control microsomes. Northern and slot blot analysis revealed that 1-benzylimidazole is effective in increasing hepatic mEH mRNA levels at 24 h post-treatment (i.e. 4-5-fold). Hepatic mEH mRNA levels were elevated from 4-4- to 8-fold following 1-benzylimidazole administration in a time-dependent manner. These results demonstrated that 1-benzylimidazole induces mEH protein in rat and that the induction is primarily associated with accumulation of mEH mRNA levels.
在大鼠中研究了1-苄基咪唑对微粒体环氧化物水解酶（mEH）基因表达的影响。免疫印迹分析表明，从以25、50或100mg/kg/天的剂量用1-苄基咪唑处理3天或6天的大鼠分离得到的肝微粒体，相对于对照微粒体，mEH水平增加了2-3倍。Northern印迹和狭缝印迹分析显示，1-苄基咪唑在处理后24小时可有效提高肝脏mEH mRNA水平（即4-5倍）。给予1-苄基咪唑后，肝脏mEH mRNA水平以时间依赖性方式从4.4倍升高至8倍。这些结果表明，1-苄基咪唑可诱导大鼠体内的mEH蛋白，且这种诱导主要与mEH mRNA水平的积累有关。
