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[The utilization of [9,10-(3)H] stearic and [1-(14)C] linolenic acids in the in-vitro synthesis of lipids in ruminant tissues].

作者信息

Zakhariv O Ia, Ianovich V G

出版信息

Zh Evol Biokhim Fiziol. 1995 Jul-Aug;31(4):404-9.

PMID:8779280
Abstract

The radioactivity of the synthesized total lipids after in vitro incubation of liver, skeletal muscle and adipose tissue homogenates from ruminants with [9,10-3H]stearic acid was 3.5, 2.9 and 6.6 times higher than after the incubation of these organs and tissues with [1-14C]linolenic acid, respectively compared with the radioactivity of non-esterificant fatty acids fraction being in this case 2.0, 1.7 and 1.6 times lower, respectively. The radioactivity of phospholipids, di- and triglycerides, free and esters cholesterols after the incubation of liver, skeletal muscle and adipose tissue homogenates with [9,10-3H]stearic acid comprised 30.6-33.5, 13.2-18.0, 23.2-32.5, 18.2-23.0, 2.8-3.3 per cent of total lipid radioactivity, respectively, and after the incubation with [1-14C]linolenic acid this correlation was as follows: 46.5-51.5,17.8-33.3, 4.0-10.6, 9.2-15.4 and 0.6-4.5 per cent of total lipids radioactivity.

摘要

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