从铜矿区土壤样品中分离并鉴定一株耐铜产甲烷菌。
Isolation and characterization of a copper-resistant methanogen from a copper-mining soil sample.
作者信息
Kim B K, Conway de Macario E, Nölling J, Daniels L
机构信息
Department of Microbiology, University of Iowa, Iowa City 52242, USA.
出版信息
Appl Environ Microbiol. 1996 Jul;62(7):2629-35. doi: 10.1128/aem.62.7.2629-2635.1996.
Abstract
A copper-resistant methanogen for which the CuSO4 MICs were approximately 2- to 36-fold higher than those for other methanogens tested was isolated from a copper-mining area in the upper peninsula of Michigan. The rod-shaped methanogen used H2-CO2 or formate, but not acetate or methanol, as a growth substrate. Standing incubation with H2-CO2 medium resulted in a mat-like surface growth, dependent on the presence of hydrogen. The presence of 1 mM cupric salt resulted in longer filamentous and intertwined cells. Antigenic fingerprinting, 16S rRNA gene analysis, morphology, and substrate use suggest that the new isolate is a novel strain of Methanobacterium bryantii that is able to use formate.
摘要
从密歇根上半岛的一个铜矿区分离出一种耐铜产甲烷菌，其对硫酸铜的最低抑菌浓度（MIC）比所测试的其他产甲烷菌高出约2至36倍。这种杆状产甲烷菌以H₂-CO₂或甲酸盐作为生长底物，而非乙酸盐或甲醇。在H₂-CO₂培养基中静置培养会形成类似垫子的表面生长物，这依赖于氢气的存在。1 mM铜盐的存在会导致细胞形成更长的丝状且相互缠绕。抗原指纹图谱、16S rRNA基因分析、形态学以及底物利用情况表明，新分离出的菌株是能够利用甲酸盐的布氏甲烷杆菌新菌株。
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